Part:BBa_K3595012:Design

up 500 bp DNA of E.coli genome in deleting argR gene

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

we connected Up-arm Down-arm and sgRNA together with vector to form gRNA plasmid that guides cas9 to cut the gene.

Source

E.coli DH10b from company Transgene.